› 3pgy

X-ray diffraction
1.92Å resolution

Serine hydroxymethyltransferase from Staphylococcus aureus, S95P mutant.

Released:
DOI: 10.2210/pdb3pgy/pdb
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PDB entry 3pgy coloured by chain, front view.
PDB entry 3pgy coloured by chain, side view.
PDB entry 3pgy coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus COL
Entry authors: Osipiuk J, Makowska-Grzyska M, Kwon K, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 415 amino acids
Theoretical weight: 45.51 KDa
Source organism: Staphylococcus aureus subsp. aureus COL
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5HE87 (Residues: 1-412; Coverage: 100%)
Gene names: SACOL2105, glyA
Sequence domains: Serine hydroxymethyltransferase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CIT 8 x CIT
Ligand GLY 3 x GLY
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 69.081Å b: 86.531Å c: 301.806Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.208
Expression system: Escherichia coli BL21(DE3)

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Experimental raw data

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Diffraction data related to PDB entry 3pgy found at the Integrated Resource for Reproducibility in Macromolecular Crystallography (IRRMC)

Data DOI: 10.18430/m33pgy
Datasets: 1
Total size: 0.87Gb
Protein diffraction thumbnail