› 3q1h

X-ray diffraction
1.8Å resolution

Crystal Structure of Dihydrofolate Reductase from Yersinia pestis

Released:
DOI: 10.2210/pdb3q1h/pdb
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PDB entry 3q1h coloured by chain, front view.
PDB entry 3q1h coloured by chain, side view.
PDB entry 3q1h coloured by chain, top view.
Source organism: Yersinia pestis CO92
Entry authors: Maltseva N, Kim Y, Makowska-Grzyska M, Mulligan R, Papazisi L, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104916 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.35 KDa
Source organism: Yersinia pestis CO92
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A3N4BLI0 (Residues: 1-160; Coverage: 100%)
Gene names: YPO0486, folA, tmrA
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 60.477Å b: 79.152Å c: 64.685Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.2
Expression system: Escherichia coli

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Experimental raw data

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Diffraction data related to PDB entry 3q1h found at the Integrated Resource for Reproducibility in Macromolecular Crystallography (IRRMC)

Data DOI: 10.18430/m33q1h
Datasets: 1
Total size: 1.39Gb
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