› 3q26

X-ray diffraction
1.54Å resolution

Cyrstal structure of human alpha-synuclein (10-42) fused to maltose binding protein (MBP)

Released:
DOI: 10.2210/pdb3q26/pdb
PDB entry 3q26 coloured by chain, front view.
PDB entry 3q26 coloured by chain, side view.
PDB entry 3q26 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of segments of α-synuclein fused to maltose-binding protein suggest intermediate states during amyloid formation.
Zhao M, Cascio D, Sawaya MR, Eisenberg D
Protein Sci 20 996-1004 (2011)
PMID: 21462277

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142535 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alpha-synuclein; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 404 amino acids
Theoretical weight: 44.02 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: P37840 (Residues: 10-42; Coverage: 24%)
Gene names: JW3994, NACP, PARK1, SNCA, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand GOL 6 x GOL
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 48.74Å b: 57.49Å c: 127.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.14 0.183
Expression system: Escherichia coli

Quick links

Citations

17 citation in other articles

A soluble α-synuclein construct forms a dynamic tetramer.
Wang et al. (2011)
16 more

162 mentions without citation

The SLC2 (GLUT) family of membrane transporters.
Mueckler et al. (2013)
161 more