› 3rl0

X-ray diffraction
3.8Å resolution

Truncated SNARE complex with complexin (P1)

Released:
DOI: 10.2210/pdb3rl0/pdb
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PDB entry 3rl0 coloured by chain, front view.
PDB entry 3rl0 coloured by chain, side view.
PDB entry 3rl0 coloured by chain, top view.
Source organisms:
Primary publication:
Complexin cross-links prefusion SNAREs into a zigzag array.
Kümmel D, Krishnakumar SS, Radoff DT, Li F, Giraudo CG, Pincet F, Rothman JE, Reinisch KM
OpenAccess logo Nat Struct Mol Biol 18 927-33 (2011)
PMID: 21785414

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-127157 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Vesicle-associated membrane protein 2 Chains: A, E, I, M, Q, U, Y, c
Molecule details ›
Chains: A, E, I, M, Q, U, Y, c
Length: 37 amino acids
Theoretical weight: 4.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63027 (Residues: 28-60; Coverage: 28%)
Gene names: SYB2, VAMP2
Sequence domains: Synaptobrevin
Syntaxin-1A Chains: B, F, J, N, R, V, Z, d
Molecule details ›
Chains: B, F, J, N, R, V, Z, d
Length: 65 amino acids
Theoretical weight: 7.55 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32851 (Residues: 191-253; Coverage: 22%)
Gene names: Sap, Stx1a
Sequence domains: SNARE domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chains: C, G, K, O, S, W, a, e
Synaptosomal-associated protein 25 Chains: D, H, L, P, T, X, b, f
Complexin-1 Chains: g, h, i, j, k, l, m, n

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 53.745Å b: 127.357Å c: 142.725Å
α: 107.49° β: 90.01° γ: 90.05°
R-values:
R R work R free
0.308 0.306 0.345
Expression system: Escherichia coli

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Citations

28 review citations

Neurotransmitter release: the last millisecond in the life of a synaptic vesicle.
Südhof TC. (2013)
27 more

5 mentions without citation

Molecular Mechanisms of Fast Neurotransmitter Release.
Brunger et al. (2018)
4 more

PDB-REDO

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