› 3rvj

X-ray diffraction
2.1Å resolution

Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89Q

Released:
DOI: 10.2210/pdb3rvj/pdb
PDB entry 3rvj coloured by chain, front view.
PDB entry 3rvj coloured by chain, side view.
PDB entry 3rvj coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Probing Mechanistic Similarities between Response Regulator Signaling Proteins and Haloacid Dehalogenase Phosphatases.
Immormino RM, Starbird CA, Silversmith RE, Bourret RB
Biochemistry 54 3514-27 (2015)
PMID: 25928369

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chemotaxis protein CheY Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 14.39 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AE67 (Residues: 1-129; Coverage: 100%)
Gene names: JW1871, b1882, cheY
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand BEF 2 x BEF
Ligand SO4 4 x SO4
Ligand GOL 12 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 53.584Å b: 53.697Å c: 160.663Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.201
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Microbial 5'-nucleotidases: their characteristics, roles in cellular metabolism, and possible practical applications.
Zakataeva NP. (2021)
1 more

2 mentions without citation

Nonconserved active site residues modulate CheY autophosphorylation kinetics and phosphodonor preference.
Thomas et al. (2013)
1 more