› 3rzt

Neutron Diffraction
1.75Å resolution

Neutron structure of perdeuterated rubredoxin using rapid (14 hours) data

Released:
DOI: 10.2210/pdb3rzt/pdb
PDB entry 3rzt coloured by chain, front view.
PDB entry 3rzt coloured by chain, side view.
PDB entry 3rzt coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Rapid visualization of hydrogen positions in protein neutron crystallographic structures.
Munshi P, Chung SL, Blakeley MP, Weiss KL, Myles DA, Meilleur F
Acta Crystallogr D Biol Crystallogr 68 35-41 (2012)
PMID: 22194331

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150200 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rubredoxin Chain: A
Molecule details ›
Chain: A
Length: 54 amino acids
Theoretical weight: 6.03 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24297 (Residues: 1-54; Coverage: 100%)
Gene names: PF1282, rub
Sequence domains: Rubredoxin
Structure domains: Rubrerythrin, domain 2

Ligands and Environments

2 bound ligands:
Ligand FE 1 x FE
Ligand DOD 35 x DOD
No modified residues

Experiments and Validation Details

Spacegroup: P212121
Unit cell:
a: 33.92Å b: 34.87Å c: 43.5Å α: 90° β: 90° γ: 90°
R-values:
R R work R free 0.204 0.202 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Neutron protein crystallography: A complementary tool for locating hydrogens in proteins.
O'Dell et al. (2016)
1 more

3 mentions without citation

Evaluation of models determined by neutron diffraction and proposed improvements to their validation and deposition.
Liebschner et al. (2018)
2 more