› 3s4d

X-ray diffraction
3.3Å resolution

Lactose phosphorylase in a ternary complex with cellobiose and sulfate

Released:
DOI: 10.2210/pdb3s4d/pdb
PDB entry 3s4d coloured by chain, front view.
PDB entry 3s4d coloured by chain, side view.
PDB entry 3s4d coloured by chain, top view.
Source organism: Cellulomonas uda
Entry authors: Van Hoorebeke A, Stout J, Soetaert W, Van Beeumen J, Desmet T, Savvides S

Function and Biology Details

Reaction catalysed: 
Cellobiose + phosphate = alpha-D-glucose 1-phosphate + D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182141 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Putative carbohydrate binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 822 amino acids
Theoretical weight: 91.5 KDa
Source organism: Cellulomonas uda
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7WTR6 (Residues: 1-822; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 84.71Å b: 92.88Å c: 104.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.297 0.296 0.34
Expression system: Escherichia coli

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