› 3sio

X-ray diffraction
2.32Å resolution

Ac-AChBP ligand binding domain (not including beta 9-10 linker) mutated to human alpha-7 nAChR

Released:
DOI: 10.2210/pdb3sio/pdb
PDB entry 3sio coloured by chain, front view.
PDB entry 3sio coloured by chain, side view.
PDB entry 3sio coloured by chain, top view.
Source organism: Aplysia californica
Primary publication:
Creating an α7 nicotinic acetylcholine recognition domain from the acetylcholine-binding protein: crystallographic and ligand selectivity analyses.
Nemecz Á, Taylor P
J Biol Chem 286 42555-42565 (2011)
PMID: 22009746

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 230 amino acids
Theoretical weight: 26.24 KDa
Source organism: Aplysia californica
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8WSF8 (Residues: 18-236; Coverage: 100%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
4 bound ligands:
Ligand NAG 7 x NAG
Ligand MLK 10 x MLK
Ligand MRD 7 x MRD
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 143.218Å b: 142.366Å c: 144.477Å
α: 90° β: 90.02° γ: 90°
R-values:
R R work R free
0.184 0.182 0.23
Expression system: Homo sapiens

Quick links

Citations

13 review citations

Structure and pharmacology of pentameric receptor channels: from bacteria to brain.
Corringer et al. (2012)
12 more

13 mentions without citation

Structure-Function of Neuronal Nicotinic Acetylcholine Receptor Inhibitors Derived From Natural Toxins.
Ho et al. (2020)
12 more