› 3tat

X-ray diffraction
3.5Å resolution

TYROSINE AMINOTRANSFERASE FROM E. COLI

Released:
DOI: 10.2210/pdb3tat/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 3tat coloured by chain, front view.
PDB entry 3tat coloured by chain, side view.
PDB entry 3tat coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystallization and preliminary crystallographic analysis of the Escherichia coli tyrosine aminotransferase.
Ko TP, Wu SP, Yang WZ, Tsai H, Yuan HS
Acta Crystallogr D Biol Crystallogr 55 1474-7 (1999)
PMID: 10417420

Function and Biology Details

Reactions catalysed: 
(2S,3S)-3-methylphenylalanine + 2-oxoglutarate = (3S)-2-oxo-3-phenylbutanoate + L-glutamate
An aromatic amino acid + 2-oxoglutarate = an aromatic oxo acid + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138148 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aromatic-amino-acid aminotransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 397 amino acids
Theoretical weight: 43.58 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P04693 (Residues: 1-397; Coverage: 100%)
Gene names: JW4014, b4054, tyrB
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 6 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P32
Unit cell:
a: 126.578Å b: 126.578Å c: 156.243Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.213 0.26
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Enzymatic asymmetric synthesis of chiral amino acids.
Xue et al. (2018)
1 more

8 mentions without citation

A Three-Ring Circus: Metabolism of the Three Proteogenic Aromatic Amino Acids and Their Role in the Health of Plants and Animals.
Parthasarathy et al. (2018)
7 more