› 3u75

X-ray diffraction
2.68Å resolution

Structure of E230A-fructofuranosidase from Schwanniomyces occidentalis complexed with fructosylnystose

Released:
DOI: 10.2210/pdb3u75/pdb
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PDB entry 3u75 coloured by chain, front view.
PDB entry 3u75 coloured by chain, side view.
PDB entry 3u75 coloured by chain, top view.
Source organism: Schwanniomyces occidentalis
Primary publication:
Structural and kinetic insights reveal that the amino acid pair Gln-228/Asn-254 modulates the transfructosylating specificity of Schwanniomyces occidentalis β-fructofuranosidase, an enzyme that produces prebiotics.
Álvaro-Benito M, Sainz-Polo MA, González-Pérez D, González B, Plou FJ, Fernández-Lobato M, Sanz-Aparicio J
J Biol Chem 287 19674-86 (2012)
PMID: 22511773

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122965 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Fructofuranosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 535 amino acids
Theoretical weight: 61.07 KDa
Source organism: Schwanniomyces occidentalis
Expression system: Escherichia coli
UniProt:
  • Canonical: E5D0X5 (Residues: 1-535; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: FRU, GLC
Carbohydrate polymer
1 bound ligand:
Ligand NAG 7 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P1
Unit cell:
a: 65.207Å b: 92.663Å c: 117.753Å
α: 89.91° β: 82.96° γ: 85.82°
R-values:
R R work R free
0.245 0.241 0.308
Expression system: Escherichia coli

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Citations

1 review citation

Oligomeric Structure of Yeast and Other Invertases Governs Specificity.
Jiménez-Ortega et al. (2024)
18 other articles

4 mentions without citation

Three-dimensional structure of Saccharomyces invertase: role of a non-catalytic domain in oligomerization and substrate specificity.
Sainz-Polo et al. (2013)
3 more

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