› 3ucw

X-ray diffraction
1.76Å resolution

Structure of MG2+ bound N-Terminal domain of Calmodulin

Released:
DOI: 10.2210/pdb3ucw/pdb
PDB entry 3ucw coloured by chain, front view.
PDB entry 3ucw coloured by chain, side view.
PDB entry 3ucw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray structures of magnesium and manganese complexes with the N-terminal domain of calmodulin: insights into the mechanism and specificity of metal ion binding to an EF-hand.
Senguen FT, Grabarek Z
Biochemistry 51 6182-94 (2012)
PMID: 22803592

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-534462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 79 amino acids
Theoretical weight: 8.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0DP23 (Residues: 2-80; Coverage: 53%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair

Ligands and Environments

1 bound ligand:
Ligand MG 10 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 34.435Å b: 43.04Å c: 53.689Å
α: 68.44° β: 88.62° γ: 79.44°
R-values:
R R work R free
0.198 0.196 0.233
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Mass Spectrometry-Based Protein Footprinting for Higher-Order Structure Analysis: Fundamentals and Applications.
Liu et al. (2020)
5 more

8 mentions without citation

Calmodulin and Ca(2+) control of voltage gated Na(+) channels.
Gabelli et al. (2016)
7 more