› 3upt

X-ray diffraction
2.4Å resolution

Crystal structure of a transketolase from Burkholderia pseudomallei bound to TPP, calcium and ribose-5-phosphate

Released:
DOI: 10.2210/pdb3upt/pdb
PDB entry 3upt coloured by chain, front view.
PDB entry 3upt coloured by chain, side view.
PDB entry 3upt coloured by chain, top view.
Source organism: Burkholderia thailandensis E264
Primary publication:
Combining functional and structural genomics to sample the essential Burkholderia structome.
Baugh L, Gallagher LA, Patrapuvich R, Clifton MC, Gardberg AS, Edwards TE, Armour B, Begley DW, Dieterich SH, Dranow DM, Abendroth J, Fairman JW, Fox D, Staker BL, Phan I, Gillespie A, Choi R, Nakazawa-Hewitt S, Nguyen MT, Napuli A, Barrett L, Buchko GW, Stacy R, Myler PJ, Stewart LJ, Manoil C, Van Voorhis WC
OpenAccess logo PLoS One 8 e53851 (2013)
PMID: 23382856

Function and Biology Details

Reaction catalysed: 
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173979 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transketolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 711 amino acids
Theoretical weight: 76.73 KDa
Source organism: Burkholderia thailandensis E264
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2SZA7 (Residues: 1-690; Coverage: 100%)
Gene names: BTH_I1195, tkt
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand TPP 2 x TPP
3 bound ligands:
Ligand CA 2 x CA
Ligand RP5 2 x RP5
Ligand BR 4 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P41212
Unit cell:
a: 146.98Å b: 146.98Å c: 142.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.151 0.2
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Prokaryotic Heme Biosynthesis: Multiple Pathways to a Common Essential Product.
Dailey et al. (2017)
10 more

2 mentions without citation

Combining functional and structural genomics to sample the essential Burkholderia structome.
Baugh et al. (2013)
1 more