› 3uyv

X-ray diffraction
2.43Å resolution

Crystal structure of a glycosylated ice-binding protein (LeIBP) from Arctic yeast

Released:
DOI: 10.2210/pdb3uyv/pdb
PDB entry 3uyv coloured by chain, front view.
PDB entry 3uyv coloured by chain, side view.
PDB entry 3uyv coloured by chain, top view.
Source organism: Leucosporidium sp. AY30
Primary publication:
Structural basis for antifreeze activity of ice-binding protein from arctic yeast.
Lee JH, Park AK, Do H, Park KS, Moh SH, Chi YM, Kim HJ
J Biol Chem 287 11460-8 (2012)
PMID: 22303017

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111697 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Ice-binding protein Chain: A
Molecule details ›
Chain: A
Length: 241 amino acids
Theoretical weight: 24.94 KDa
Source organism: Leucosporidium sp. AY30
Expression system: Komagataella pastoris
UniProt:
  • Canonical: C7F6X3 (Residues: 21-261; Coverage: 100%)
Gene name: AFP
Sequence domains: Ice-binding-like

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P6522
Unit cell:
a: 58.64Å b: 58.64Å c: 292.641Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.233 0.286
Expression system: Komagataella pastoris

Quick links

Citations

7 review citations

Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant.
Kim et al. (2017)
6 more

3 mentions without citation

Ice Binding Proteins: Diverse Biological Roles and Applications in Different Types of Industry.
Białkowska et al. (2020)
2 more