› 3vax

X-ray diffraction
2.4Å resolution

Crystal structure of DndA from streptomyces lividans

Released:
DOI: 10.2210/pdb3vax/pdb
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Source organism: Streptomyces lividans
Primary publication:
Crystal structure of the cysteine desulfurase DndA from Streptomyces lividans which is involved in DNA phosphorothioation.
Chen F, Zhang Z, Lin K, Qian T, Zhang Y, You D, He X, Wang Z, Liang J, Deng Z, Wu G
OpenAccess logo PLoS One 7 e36635 (2012)
PMID: 22570733

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-108399 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cysteine desulfurase Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 43.29 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: A7TUX7 (Residues: 19-397; Coverage: 96%)
Gene names: SLG80, dndA
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P2
Unit cell:
a: 77.9Å b: 67.28Å c: 85.56Å
α: 90° β: 97.04° γ: 90°
R-values:
R R work R free
0.194 0.192 0.234
Expression system: Escherichia coli

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Citations

3 review citations

Comparative genomics of defense systems in archaea and bacteria.
Makarova et al. (2013)
2 more

3 mentions without citation

Crystal structure of the cysteine desulfurase DndA from Streptomyces lividans which is involved in DNA phosphorothioation.
Chen et al. (2012)
2 more

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