› 3vdz

X-ray diffraction
2.4Å resolution

Tailoring Encodable Lanthanide-Binding Tags as MRI Contrast Agents: xq-dSE3-Ubiquitin at 2.4 Angstroms

Released:
DOI: 10.2210/pdb3vdz/pdb
PDB entry 3vdz coloured by chain, front view.
PDB entry 3vdz coloured by chain, side view.
PDB entry 3vdz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tailoring encodable lanthanide-binding tags as MRI contrast agents.
Daughtry KD, Martin LJ, Sarraju A, Imperiali B, Allen KN
Chembiochem 13 2567-74 (2012)
PMID: 23150430

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158832 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chains: A, B
Molecule details ›
Chains: A, B
Length: 111 amino acids
Theoretical weight: 12.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62987 (Residues: 1-76; Coverage: 59%)
Gene names: UBA52, UBCEP2
Sequence domains: Ubiquitin family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GD 4 x GD
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P32
Unit cell:
a: 46.166Å b: 46.166Å c: 113.552Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.223 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Metalloprotein-based MRI probes.
Matsumoto et al. (2013)
7 other articles