› 3w7t

X-ray diffraction
1.5Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154828 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosidase YgjK Chains: A, B
Molecule details ›
Chains: A, B
Length: 760 amino acids
Theoretical weight: 86 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P42592 (Residues: 24-783; Coverage: 100%)
Gene names: JW3051, b3080, ygjK
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand MG 2 x MG
Ligand BMA 7 x BMA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P21
Unit cell:
a: 62.467Å b: 137.844Å c: 86.524Å
α: 90° β: 98.07° γ: 90°
R-values:
R R work R free
0.157 0.154 0.18
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Calcium binding proteins and calcium signaling in prokaryotes.
Domínguez et al. (2015)
4 more

1 mention without citation

Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM.
Uchański et al. (2021)