› 3wbe

X-ray diffraction
1.97Å resolution

Rice Os3BGlu6 Beta-Glucosidase E178Q mutant in a covalent complex with Glc from GA4GE.

Released:
DOI: 10.2210/pdb3wbe/pdb
PDB entry 3wbe coloured by chain, front view.
PDB entry 3wbe coloured by chain, side view.
PDB entry 3wbe coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Enzymatic and structural characterization of hydrolysis of gibberellin A4 glucosyl ester by a rice β-D-glucosidase.
Hua Y, Sansenya S, Saetang C, Wakuta S, Ketudat Cairns JR
Arch Biochem Biophys 537 39-48 (2013)
PMID: 23811195

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-569299 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase 6 Chain: A
Molecule details ›
Chain: A
Length: 489 amino acids
Theoretical weight: 55.37 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8L7J2 (Residues: 38-521; Coverage: 100%)
Gene names: BGLU6, LOC_Os03g11420, Os03g0212800
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand GLC 1 x GLC
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 57.047Å b: 91.215Å c: 111.368Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.145 0.18
Expression system: Escherichia coli

Quick links

Citations

1 review citation

β-Glucosidases: Multitasking, moonlighting or simply misunderstood?
Ketudat Cairns et al. (2015)
7 other articles