› 3wky

X-ray diffraction
1.8Å resolution

Crystal structure of hemolymph type prophenoloxidase (proPOb) from crustacean

Released:
DOI: 10.2210/pdb3wky/pdb
PDB entry 3wky coloured by chain, front view.
PDB entry 3wky coloured by chain, side view.
PDB entry 3wky coloured by chain, top view.
Source organism: Penaeus japonicus
Primary publication:
The crystal structure of a crustacean prophenoloxidase provides a clue to understanding the functionality of the type 3 copper proteins.
Masuda T, Momoji K, Hirata T, Mikami B
FEBS J 281 2659-73 (2014)
PMID: 24720693

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124531 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Tyrosinase copper-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 687 amino acids
Theoretical weight: 78.9 KDa
Source organism: Penaeus japonicus
UniProt:
  • Canonical: G5EKM4 (Residues: 22-708; Coverage: 100%)
Gene name: proPOb
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
6 bound ligands:
Ligand NAG 10 x NAG
Ligand CUO 2 x CUO
Ligand EDO 9 x EDO
Ligand NA 1 x NA
Ligand CL 5 x CL
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: R3
Unit cell:
a: 156.706Å b: 156.706Å c: 283.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.175 0.196

Quick links

Citations

2 review citations

Shrimp humoral responses against pathogens: antimicrobial peptides and melanization.
Tassanakajon et al. (2018)
1 more

7 mentions without citation

Structure-function correlations in tyrosinases.
Kanteev et al. (2015)
6 more