› 3x32

X-ray diffraction
0.83Å resolution

Crystal structure of the oxidized form of the solubilized domain of porcine cytochrome b5 in form 1 crystal

Released:
DOI: 10.2210/pdb3x32/pdb
PDB entry 3x32 coloured by chain, front view.
PDB entry 3x32 coloured by chain, side view.
PDB entry 3x32 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
High-resolution crystal structures of the solubilized domain of porcine cytochrome b5.
Hirano Y, Kimura S, Tamada T
OpenAccess logo Acta Crystallogr D Biol Crystallogr 71 1572-81 (2015)
PMID: 26143928

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132304 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome b5 Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 10.77 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00172 (Residues: 1-94; Coverage: 70%)
Gene names: CYB5, CYB5A
Sequence domains: Cytochrome b5-like Heme/Steroid binding domain
Structure domains: Cytochrome b5-like heme/steroid binding domain

Ligands and Environments

3 bound ligands:
Ligand HEM 1 x HEM
Ligand CA 2 x CA
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 32.339Å b: 39.279Å c: 60.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.109 0.106 0.127
Expression system: Escherichia coli BL21

Quick links

Citations

1 citations in other articles

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Gui et al. (2021)

4 mentions without citation

Comparison of structure- and ligand-based scoring functions for deep generative models: a GPCR case study.
Thomas et al. (2021)
3 more