› 3zew

X-ray diffraction
2.5Å resolution

Crystal structure of EphB4 in complex with staurosporine

Released:
DOI: 10.2210/pdb3zew/pdb
PDB entry 3zew coloured by chain, front view.
PDB entry 3zew coloured by chain, side view.
PDB entry 3zew coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Completing the structural family portrait of the human EphB tyrosine kinase domains.
Overman RC, Debreczeni JE, Truman CM, McAlister MS, Attwood TK
Protein Sci 23 627-38 (2014)
PMID: 24677421

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-547416 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ephrin type-B receptor 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 298 amino acids
Theoretical weight: 33.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54760 (Residues: 598-892; Coverage: 30%)
Gene names: EPHB4, HTK, MYK1, TYRO11
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand STU 2 x STU
Ligand SO4 4 x SO4
1 modified residue:
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 52.17Å b: 88.71Å c: 80.78Å
α: 90° β: 103.05° γ: 90°
R-values:
R R work R free
0.24 0.238 0.282
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Factors involved in heterologous expression of proteins in E. coli host.
Pouresmaeil et al. (2023)
1 more

6 mentions without citation

Completing the structural family portrait of the human EphB tyrosine kinase domains.
Overman et al. (2014)
5 more