› 3zle

X-ray diffraction
2.35Å resolution

Crystal structure of Toxoplasma gondii sporozoite AMA1

Released:
DOI: 10.2210/pdb3zle/pdb
PDB entry 3zle coloured by chain, front view.
PDB entry 3zle coloured by chain, side view.
PDB entry 3zle coloured by chain, top view.
Source organism: Toxoplasma gondii
Primary publication:
Toxoplasma gondii sporozoites invade host cells using two novel paralogues of RON2 and AMA1.
Poukchanski A, Fritz HM, Tonkin ML, Treeck M, Boulanger MJ, Boothroyd JC
OpenAccess logo PLoS One 8 e70637 (2013)
PMID: 23940612

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110350 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apical membrane antigen 1-like protein, soluble form Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 396 amino acids
Theoretical weight: 42.82 KDa
Source organism: Toxoplasma gondii
Expression system: Trichoplusia ni
UniProt:
  • Canonical: B6K9M7 (Residues: 97-480; Coverage: 63%)
Gene name: TGVEG_315730
Sequence domains: Apical membrane antigen 1
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NAG 9 x NAG
Ligand GOL 18 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 179.19Å b: 155.52Å c: 180.59Å
α: 90° β: 92.31° γ: 90°
R-values:
R R work R free
0.21 0.209 0.244
Expression system: Trichoplusia ni

Quick links

Citations

7 review citations

Host cell invasion by apicomplexan parasites: the junction conundrum.
Bargieri et al. (2014)
6 more

2 mentions without citation

The apicomplexan glideosome and adhesins - Structures and function.
Boucher et al. (2015)
1 more