› 3zxp

X-ray diffraction
2.5Å resolution

Structural and Functional Analyses of the Bro1 Domain Protein BROX

Released:
DOI: 10.2210/pdb3zxp/pdb
PDB entry 3zxp coloured by chain, front view.
PDB entry 3zxp coloured by chain, side view.
PDB entry 3zxp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the Bro1 domain protein BROX and functional analyses of the ALIX Bro1 domain in HIV-1 budding.
Zhai Q, Landesman MB, Robinson H, Sundquist WI, Hill CP
OpenAccess logo PLoS One 6 e27466 (2011)
PMID: 22162750

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-562667 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BRO1 domain-containing protein BROX Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 407 amino acids
Theoretical weight: 46.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5VW32 (Residues: 1-401; Coverage: 98%)
Gene names: BROFTI, BROX, C1orf58
Sequence domains: BRO1-like domain
Structure domains: alix/aip1 like domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 228.064Å b: 67.164Å c: 103.865Å
α: 90° β: 96.95° γ: 90°
R-values:
R R work R free
0.204 0.201 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Membrane fission reactions of the mammalian ESCRT pathway.
McCullough et al. (2013)
6 more

1 mention without citation

Structure of the Bro1 domain protein BROX and functional analyses of the ALIX Bro1 domain in HIV-1 budding.
Zhai et al. (2011)