› 3a1k

X-ray diffraction
2.17Å resolution

Crystal structure of Rhodococcus sp. N771 Amidase

Released:
DOI: 10.2210/pdb3a1k/pdb
PDB entry 3a1k coloured by chain, front view.
PDB entry 3a1k coloured by chain, side view.
PDB entry 3a1k coloured by chain, top view.
Source organism: Rhodococcus sp. N-771
Primary publication:
Structure and characterization of amidase from Rhodococcus sp. N-771: Insight into the molecular mechanism of substrate recognition.
Ohtaki A, Murata K, Sato Y, Noguchi K, Miyatake H, Dohmae N, Yamada K, Yohda M, Odaka M
Biochim Biophys Acta 1804 184-92 (2010)
PMID: 19819352

Function and Biology Details

Reaction catalysed: 
A monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-181501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 521 amino acids
Theoretical weight: 54.76 KDa
Source organism: Rhodococcus sp. N-771
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7DKE4 (Residues: 1-521; Coverage: 100%)
Gene name: ami
Sequence domains: Amidase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P41212
Unit cell:
a: 92.672Å b: 92.672Å c: 162.305Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.232
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Transfer and degradation of polyacrylamide-based flocculants in hydrosystems: a review.
Guezennec et al. (2015)
1 more

3 mentions without citation

Discovery of a novel (+)-γ-lactamase from Bradyrhizobium japonicum USDA 6 by rational genome mining.
Zhu et al. (2012)
2 more