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Ser386 phosphorylation of transcription factor IRF-3 induces dimerization and association with CBP/p300 without overall conformational change.

Takahasi K, Horiuchi M, Fujii K, Nakamura S, Noda NN, Yoneyama M, Fujita T, Inagaki F
Genes Cells 15 901-10 (2010)
Cited: 46 times
EuropePMC logo PMID: 20604809

Abstract

The transcription factor IRF-3 is activated by microbial invasions and produces a variety of cytokines including type-I interferon. Upon microbial infection, IRF-3 is phosphorylated at its C-terminal regulatory domain, then oligomerized, translocated into the nucleus, and here it binds to CBP/p300. Although a number of studies have been reported investigating the activation mechanism of IRF-3, there are a number of unresolved issues, especially on the phosphorylation sites, the oligomerization process and the binding mechanism with CBP/p300. In this report, the phosphorylated IRF-3 regulatory domain (IRF-3 RD) was prepared using the kinase IKK-i, and the active form of phosphorylated IRF-3 RD was identified. The paper also reports the crystal structure of the active form of the phosphorylated IRF-3 RD. Furthermore, the phosphorylation of Ser386 was found to be essential for its dimerization and binding with CBP/p300 using mutational analysis and mass spectrometry. Thus, we conclude that the phosphorylation of Ser386 is essential for activation of IRF-3.

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  1. Interferon Regulatory Factor 9 Structure and Regulation. Paul A, Tang TH, Ng SK. Front Immunol 9 1831 (2018)

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  2. Ligand-induced Ordering of the C-terminal Tail Primes STING for Phosphorylation by TBK1. Tsuchiya Y, Jounai N, Takeshita F, Ishii KJ, Mizuguchi K. EBioMedicine 9 87-96 (2016)
  3. Emodin regulates neutrophil phenotypes to prevent hypercoagulation and lung carcinogenesis. Li Z, Lin Y, Zhang S, Zhou L, Yan G, Wang Y, Zhang M, Wang M, Lin H, Tong Q, Duan Y, Du G. J Transl Med 17 90 (2019)
  4. DROIDS 1.20: A GUI-Based Pipeline for GPU-Accelerated Comparative Protein Dynamics. Babbitt GA, Mortensen JS, Coppola EE, Adams LE, Liao JK. Biophys J 114 1009-1017 (2018)
  5. The dipeptidyl peptidase IV inhibitors vildagliptin and K-579 inhibit a phospholipase C: a case of promiscuous scaffolds in proteins. Chakraborty S, Rendón-Ramírez A, Ásgeirsson B, Dutta M, Ghosh AS, Oda M, Venkatramani R, Rao BJ, Dandekar AM, Goñi FM. F1000Res 2 286 (2013)


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  1. Direct Inhibition of IRF-Dependent Transcriptional Regulatory Mechanisms Associated With Disease. Antonczyk A, Krist B, Sajek M, Michalska A, Piaszyk-Borychowska A, Plens-Galaska M, Wesoly J, Bluyssen HAR. Front Immunol 10 1176 (2019)
  2. Structural Basis of Intracellular TGF-β Signaling: Receptors and Smads. Chaikuad A, Bullock AN. Cold Spring Harb Perspect Biol 8 a022111 (2016)
  3. Regulation of antiviral innate immune signaling and viral evasion following viral genome sensing. Chathuranga K, Weerawardhana A, Dodantenna N, Lee JS. Exp Mol Med 53 1647-1668 (2021)
  4. The role of TBK1 in cancer pathogenesis and anticancer immunity. Runde AP, Mack R, S J PB, Zhang J. J Exp Clin Cancer Res 41 135 (2022)
  5. Of Keeping and Tipping the Balance: Host Regulation and Viral Modulation of IRF3-Dependent IFNB1 Expression. Schwanke H, Stempel M, Brinkmann MM. Viruses 12 E733 (2020)
  6. Viral recognition and the antiviral interferon response. Dalskov L, Gad HH, Hartmann R. EMBO J 42 e112907 (2023)
  7. Protein Dynamics in Cytosolic DNA-Sensing Antiviral Innate Immune Signaling Pathways. Zheng C. Front Immunol 11 1255 (2020)
  8. Strategies to Circumvent Host Innate Immune Response by Hepatitis C Virus. Patra T, Ray RB, Ray R. Cells 8 E274 (2019)
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  2. Endoplasmic reticulum stress regulates the innate immunity critical transcription factor IRF3. Liu YP, Zeng L, Tian A, Bomkamp A, Rivera D, Gutman D, Barber GN, Olson JK, Smith JA. J Immunol 189 4630-4639 (2012)
  3. Structural basis for concerted recruitment and activation of IRF-3 by innate immune adaptor proteins. Zhao B, Shu C, Gao X, Sankaran B, Du F, Shelton CL, Herr AB, Ji JY, Li P. Proc Natl Acad Sci U S A 113 E3403-12 (2016)
  4. SARS-CoV-2 Nucleocapsid Protein Targets RIG-I-Like Receptor Pathways to Inhibit the Induction of Interferon Response. Oh SJ, Shin OS. Cells 10 530 (2021)
  5. Structural insights into the functions of TBK1 in innate antimicrobial immunity. Shu C, Sankaran B, Chaton CT, Herr AB, Mishra A, Peng J, Li P. Structure 21 1137-1148 (2013)
  6. Degradation of CREB-binding protein and modulation of type I interferon induction by the zinc finger motif of the porcine reproductive and respiratory syndrome virus nsp1α subunit. Han M, Du Y, Song C, Yoo D. Virus Res 172 54-65 (2013)
  7. H5N1 influenza virus-induced mediators upregulate RIG-I in uninfected cells by paracrine effects contributing to amplified cytokine cascades. Hui KP, Lee SM, Cheung CY, Mao H, Lai AK, Chan RW, Chan MC, Tu W, Guan Y, Lau YL, Peiris JS. J Infect Dis 204 1866-1878 (2011)
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  9. Complex Regulation Pattern of IRF3 Activation Revealed by a Novel Dimerization Reporter System. Wang Z, Ji J, Peng D, Ma F, Cheng G, Qin FX. J Immunol 196 4322-4330 (2016)
  10. Nuclear export signal of PRRSV NSP1α is necessary for type I IFN inhibition. Chen Z, Liu S, Sun W, Chen L, Yoo D, Li F, Ren S, Guo L, Cong X, Li J, Zhou S, Wu J, Du Y, Wang J. Virology 499 278-287 (2016)
  11. Regulation of cooperative function of the Il12b enhancer and promoter by the interferon regulatory factors 3 and 5. Koshiba R, Yanai H, Matsuda A, Goto A, Nakajima A, Negishi H, Nishio J, Smale ST, Taniguchi T. Biochem Biophys Res Commun 430 95-100 (2013)
  12. AGO2 Negatively Regulates Type I Interferon Signaling Pathway by Competition Binding IRF3 with CBP/p300. Wang S, Sun X, Yi C, Zhang D, Lin X, Sun X, Chen H, Jin M. Front Cell Infect Microbiol 7 195 (2017)
  13. Epstein-Barr Virus Early Protein BFRF1 Suppresses IFN-β Activity by Inhibiting the Activation of IRF3. Wang P, Deng Y, Guo Y, Xu Z, Li Y, Ou X, Xie L, Lu M, Zhong J, Li B, Hu L, Deng S, Peng T, Cai M, Li M. Front Immunol 11 513383 (2020)
  14. The crystal structure of the DNA-binding domain of vIRF-1 from the oncogenic KSHV reveals a conserved fold for DNA binding and reinforces its role as a transcription factor. Hew K, Dahlroth SL, Venkatachalam R, Nasertorabi F, Lim BT, Cornvik T, Nordlund P. Nucleic Acids Res 41 4295-4306 (2013)
  15. A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3. Robitaille AC, Mariani MK, Fortin A, Grandvaux N. J Vis Exp e53723 (2016)
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  18. Clarithromycin prevents human respiratory syncytial virus-induced airway epithelial responses by modulating activation of interferon regulatory factor-3. Yamamoto K, Yamamoto S, Ogasawara N, Takano K, Shiraishi T, Sato T, Miyata R, Kakuki T, Kamekura R, Kojima T, Tsutsumi H, Himi T, Yokota SI. Pharmacol Res 111 804-814 (2016)
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  21. DDX56 inhibits type I interferon by disrupting assembly of IRF3-IPO5 to inhibit IRF3 nucleus import. Li D, Fu S, Wu Z, Yang W, Ru Y, Shu H, Liu X, Zheng H. J Cell Sci 133 jcs230409 (2019)
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  23. Nucleotide receptor P2RX7 stimulation enhances LPS-induced interferon-β production in murine macrophages. Gavala ML, Liu YP, Lenertz LY, Zeng L, Blanchette JB, Guadarrama AG, Denlinger LC, Bertics PJ, Smith JA. J Leukoc Biol 94 759-768 (2013)
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  25. The RNA helicase DDX3 promotes IFNB transcription via enhancing IRF-3/p300 holocomplex binding to the IFNB promoter. Saikruang W, Ang Yan Ping L, Abe H, Kasumba DM, Kato H, Fujita T. Sci Rep 12 3967 (2022)
  26. Adaptations of Interferon Regulatory Factor 3 with Transition from Terrestrial to Aquatic Life. Angeletti M, Hsu WN, Majo N, Moriyama H, Moriyama EN, Zhang L. Sci Rep 10 4508 (2020)
  27. High-resolution kinetic characterization of the RIG-I-signaling pathway and the antiviral response. Burkart SS, Schweinoch D, Frankish J, Sparn C, Wüst S, Urban C, Merlo M, Magalhães VG, Piras A, Pichlmair A, Willemsen J, Kaderali L, Binder M. Life Sci Alliance 6 e202302059 (2023)
  28. DEAD-box RNA helicase 21 negatively regulates cytosolic RNA-mediated innate immune signaling. Li J, Fang P, Zhou Y, Wang D, Fang L, Xiao S. Front Immunol 13 956794 (2022)
  29. Endothelial deletion of TBK1 contributes to BRB dysfunction via CXCR4 phosphorylation suppression. Zhao B, Ni Y, Zhang H, Zhao Y, Li L. Cell Death Discov 8 429 (2022)
  30. Hypothiocyanous Acid Suppresses PolyI:C-Induced Antiviral Responses by Modulating IRF3 Phosphorylation in Human Airway Epithelial Cells. Nguyen TT, Suzuki S, Sugamata R, Ito F, Tran DH, Yamamoto T, Kawachi S, Suzuki K. Tohoku J Exp Med 245 131-140 (2018)
  31. Letter Unraveling the molecular details of the innate immune response. Fernández-Recio J. EBioMedicine 9 7-8 (2016)


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