› 3a7n

X-ray diffraction
1.95Å resolution

Crystal structure of uracil-DNA glycosylase from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb3a7n/pdb
PDB entry 3a7n coloured by chain, front view.
PDB entry 3a7n coloured by chain, side view.
PDB entry 3a7n coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structure of uracil-DNA glycosylase from Mycobacterium tuberculosis: insights into interactions with ligands.
Kaushal PS, Talawar RK, Varshney U, Vijayan M
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 887-92 (2010)
PMID: 20693660

Function and Biology Details

Reaction catalysed: 
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 25.81 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WFQ9 (Residues: 1-227; Coverage: 100%)
Gene names: MTCY349.11, Rv2976c, ung
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain

Ligands and Environments

1 bound ligand:
Ligand FLC 2 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 44.84Å b: 63.67Å c: 86.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.199
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Structure of uracil-DNA glycosylase from Mycobacterium tuberculosis: insights into interactions with ligands.
Kaushal et al. (2010)