› 3acz

X-ray diffraction
1.97Å resolution

Crystal structure of Entamoeba histolytica methionine gamma-lyase 1

Released:
DOI: 10.2210/pdb3acz/pdb
PDB entry 3acz coloured by chain, front view.
PDB entry 3acz coloured by chain, side view.
PDB entry 3acz coloured by chain, top view.
Source organism: Entamoeba histolytica
Entry authors: Karaki T, Sato D, Shimizu A, Nozaki T, Harada S

Function and Biology Details

Reaction catalysed: 
(1a) L-methionine = methanethiol + 2-aminobut-2-enoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine gamma-lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 389 amino acids
Theoretical weight: 42.58 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86D28 (Residues: 1-389; Coverage: 100%)
Gene name: metg
Sequence domains: Cys/Met metabolism PLP-dependent enzyme
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 12 x SO4
Ligand GOL 2 x GOL
1 modified residue:
Ligand LLP 4 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 99.285Å b: 85.336Å c: 114.62Å
α: 90° β: 101.96° γ: 90°
R-values:
R R work R free
0.157 0.156 0.19
Expression system: Escherichia coli

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