› 3aod

X-ray diffraction
3.3Å resolution

Structures of the multidrug exporter AcrB reveal a proximal multisite drug-binding pocket

Released:
DOI: 10.2210/pdb3aod/pdb
PDB entry 3aod coloured by chain, front view.
PDB entry 3aod coloured by chain, side view.
PDB entry 3aod coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structures of the multidrug exporter AcrB reveal a proximal multisite drug-binding pocket.
Nakashima R, Sakurai K, Yamasaki S, Nishino K, Yamaguchi A
Nature 480 565-9 (2011)
PMID: 22121023

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug efflux pump subunit AcrB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1053 amino acids
Theoretical weight: 114.22 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MIY 1 x MIY
Ligand RFP 1 x RFP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 226.604Å b: 134.496Å c: 162.833Å
α: 90° β: 97.75° γ: 90°
R-values:
R R work R free
0.261 0.258 0.318
Expression system: Escherichia coli

Quick links

Citations

42 review citations

Molecular mechanisms of antibiotic resistance.
Blair et al. (2015)
41 more

12 mentions without citation

Ever-Adapting RND Efflux Pumps in Gram-Negative Multidrug-Resistant Pathogens: A Race against Time.
Zwama et al. (2021)
11 more