› 3apy

X-ray diffraction
2.8Å resolution

Properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb3apy/pdb
PDB entry 3apy coloured by chain, front view.
PDB entry 3apy coloured by chain, side view.
PDB entry 3apy coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8.
Igari S, Ohtaki A, Yamanaka Y, Sato Y, Yohda M, Odaka M, Noguchi K, Yamada K
OpenAccess logo PLoS One 6 e23716 (2011)
PMID: 21858212

Function and Biology Details

Reaction catalysed: 
5-methyltetrahydrofolate + NAD(+) = 5,10-methylenetetrahydrofolate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178167 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylenetetrahydrofolate reductase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 310 amino acids
Theoretical weight: 34.65 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SLG6 (Residues: 1-296; Coverage: 100%)
Gene name: TTHA0327
Sequence domains: Methylenetetrahydrofolate reductase
Structure domains: TIM Barrel

Ligands and Environments

2 bound ligands:
Ligand FAD 8 x FAD
Ligand CL 6 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 116.588Å b: 90.93Å c: 125.152Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.204 0.306
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Analysis of the Core Genome and Pan-Genome of Autotrophic Acetogenic Bacteria.
Shin et al. (2016)
3 more

4 mentions without citation

Properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8.
Igari et al. (2011)
3 more