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Crystal structure of TcOYE with pHBA

X-ray diffraction
2.04Å resolution
PDB entry 3atz coloured by chain, front view.
PDB entry 3atz coloured by chain, side view.
PDB entry 3atz coloured by chain, top view.
Source organism: Trypanosoma cruzi
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 13309.03 Å2
Buried surface area: 1239.94 Å2
Dissociation area: 220.3 Å2
Dissociation energy (ΔGdiss): -7.07 kcal/mol
Dissociation entropy (TΔSdiss): 1.69 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-184702
Assembly 2
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Assembly Name: NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 13399.76 Å2
Buried surface area: 1250.5 Å2
Dissociation area: 224.91 Å2
Dissociation energy (ΔGdiss): -6.96 kcal/mol
Dissociation entropy (TΔSdiss): 1.69 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-184702
Assembly 3
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Assembly Name: NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 13742.87 Å2
Buried surface area: 1263.3 Å2
Dissociation area: 222.83 Å2
Dissociation energy (ΔGdiss): -6.94 kcal/mol
Dissociation entropy (TΔSdiss): 1.69 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-184702
Assembly 4
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Assembly Name: NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 13679.44 Å2
Buried surface area: 1261.04 Å2
Dissociation area: 224.5 Å2
Dissociation energy (ΔGdiss): -7.21 kcal/mol
Dissociation entropy (TΔSdiss): 1.69 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-184702

Macromolecules

NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein
Chains: A, B, C, D
Length: 379 amino acids
Theoretical weight: 42.31 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8I6L9 (Residues: 1-379; Coverage: 100%)
Gene names: ECC02_012910, ECC02_013670, TcPGFS
Pfam: NADH:flavin oxidoreductase / NADH oxidase family
InterPro:
CATH: Aldolase class I
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein in PDB entry 3atz, assembly 1, front view.
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein in PDB entry 3atz, assembly 1, side view.
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein in PDB entry 3atz, assembly 1, top view.
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