› 3b0g

X-ray diffraction
1.25Å resolution

Assimilatory nitrite reductase (Nii3) from tobbaco leaf

Released:
DOI: 10.2210/pdb3b0g/pdb
PDB entry 3b0g coloured by chain, front view.
PDB entry 3b0g coloured by chain, side view.
PDB entry 3b0g coloured by chain, top view.
Source organism: Nicotiana tabacum
Primary publication:
Structure-function relationship of assimilatory nitrite reductases from the leaf and root of tobacco based on high-resolution structures.
Nakano S, Takahashi M, Sakamoto A, Morikawa H, Katayanagi K
Protein Sci 21 383-95 (2012)
PMID: 22238192

Function and Biology Details

Reaction catalysed: 
NH(3) + 2 H(2)O + 6 oxidized ferredoxin = nitrite + 6 reduced ferredoxin + 7 H(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181164 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin--nitrite reductase, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 591 amino acids
Theoretical weight: 66.46 KDa
Source organism: Nicotiana tabacum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q76KB0 (Residues: 19-587; Coverage: 97%)
Gene name: nii3
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SRM 1 x SRM
3 bound ligands:
Ligand SF4 1 x SF4
Ligand K 1 x K
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P4212
Unit cell:
a: 134.051Å b: 134.051Å c: 77.801Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.16 0.168
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Heme in the marine environment: from cells to the iron cycle.
Hogle et al. (2014)
3 other articles

2 mentions without citation

The iron-sulfur cluster assembly machineries in plants: current knowledge and open questions.
Couturier et al. (2013)
1 more