› 3b38

X-ray diffraction
1.85Å resolution

Structure of A104V DJ-1

Released:
DOI: 10.2210/pdb3b38/pdb
PDB entry 3b38 coloured by chain, front view.
PDB entry 3b38 coloured by chain, side view.
PDB entry 3b38 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural impact of three Parkinsonism-associated missense mutations on human DJ-1.
Lakshminarasimhan M, Maldonado MT, Zhou W, Fink AL, Wilson MA
Biochemistry 47 1381-92 (2008)
PMID: 18181649

Function and Biology Details

Reaction catalysed: 
An N(6)-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H(2)O = a [protein]-L-lysine + lactate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Parkinson disease protein 7 Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 20.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99497 (Residues: 1-189; Coverage: 100%)
Gene name: PARK7
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand EDO 7 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P6522
Unit cell:
a: 66.842Å b: 66.842Å c: 175.852Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.181 0.226
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Oxidative stress: A major pathogenesis and potential therapeutic target of antioxidative agents in Parkinson's disease and Alzheimer's disease.
Jiang et al. (2016)
6 more

1 mention without citation

Structural impact of three Parkinsonism-associated missense mutations on human DJ-1.
Lakshminarasimhan et al. (2008)