› 3bli

X-ray diffraction
2.5Å resolution

Crystal structure of the catalytic domain of LiCMS in complexed with pyruvate and acetyl-CoA

Released:
DOI: 10.2210/pdb3bli/pdb
PDB entry 3bli coloured by chain, front view.
PDB entry 3bli coloured by chain, side view.
PDB entry 3bli coloured by chain, top view.
Source organism: Leptospira interrogans
Primary publication:
Molecular basis of the substrate specificity and the catalytic mechanism of citramalate synthase from Leptospira interrogans.
Ma J, Zhang P, Zhang Z, Zha M, Xu H, Zhao G, Ding J
Biochem J 415 45-56 (2008)
PMID: 18498255

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-568489 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(R)-citramalate synthase CimA Chain: A
Molecule details ›
Chain: A
Length: 337 amino acids
Theoretical weight: 37.61 KDa
Source organism: Leptospira interrogans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8F3Q1 (Residues: 1-325; Coverage: 63%)
Gene names: LA_2350, cimA
Sequence domains:
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand ACO 1 x ACO
Ligand PYR 1 x PYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU ULTRAX 18
Spacegroup: P3121
Unit cell:
a: 84.99Å b: 84.99Å c: 115.73Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.229 0.281
Expression system: Escherichia coli

Quick links

Citations

11 citation in other articles

Atypical OmpR/PhoB subfamily response regulator GlnR of actinomycetes functions as a homodimer, stabilized by the unphosphorylated conserved Asp-focused charge interactions.
Lin et al. (2014)
10 more

1 mention without citation

Insight into de-regulation of amino acid feedback inhibition: a focus on structure analysis method.
Naz et al. (2023)