3bsx

X-ray diffraction
2.32Å resolution

Crystal Structure of Human Pumilio 1 in complex with Puf5 RNA

Released:
Model geometry
Fit model/data

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-172067 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Pumilio homolog 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 343 amino acids
Theoretical weight: 39.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14671 (Residues: 828-1170; Coverage: 29%)
Gene names: KIAA0099, PUM1, PUMH1
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant
5'-R(*UP*UP*GP*UP*AP*AP*UP*AP*UP*UP*A)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 nucleotides
Theoretical weight: 3.45 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P212121
Unit cell:
a: 35.835Å b: 65.642Å c: 313.975Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.212 0.277
Expression systems:
  • Escherichia coli
  • Not provided