› 3bts

X-ray diffraction
2.7Å resolution

Crystal structure of a ternary complex of the transcriptional repressor Gal80p (Gal80S0 [G301R]) and the acidic activation domain of Gal4p (aa 854-874) from Saccharomyces cerevisiae with NAD

Released:
DOI: 10.2210/pdb3bts/pdb
PDB entry 3bts coloured by chain, front view.
PDB entry 3bts coloured by chain, side view.
PDB entry 3bts coloured by chain, top view.
Source organisms:
Primary publication:
NADP regulates the yeast GAL induction system.
Kumar PR, Yu Y, Sternglanz R, Johnston SA, Joshua-Tor L
Science 319 1090-2 (2008)
PMID: 18292341

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-528273 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Galactose/lactose metabolism regulatory protein GAL80 Chains: A, B
Molecule details ›
Chains: A, B
Length: 438 amino acids
Theoretical weight: 48.75 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P04387 (Residues: 1-435; Coverage: 100%)
Gene names: GAL80, YM9827.01, YM9958.12, YML051W
Sequence domains:
Structure domains:
Regulatory protein GAL4 Chains: E, F
Molecule details ›
Chains: E, F
Length: 21 amino acids
Theoretical weight: 2.51 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P04386 (Residues: 854-874; Coverage: 2%)
Gene names: GAL4, YPL248C

Ligands and Environments

1 bound ligand:
Ligand NAD 2 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 88.071Å b: 103.431Å c: 106.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.175 0.232
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Nutrient sensing and signaling in the yeast Saccharomyces cerevisiae.
Conrad et al. (2014)
6 more

3 mentions without citation

The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation.
Lavy et al. (2012)
2 more