› 3bz8

X-ray diffraction
2.2Å resolution

Crystal Structures of (S)-(-)-Blebbistatin Analogs bound to Dictyostelium discoideum myosin II

Released:
DOI: 10.2210/pdb3bz8/pdb
PDB entry 3bz8 coloured by chain, front view.
PDB entry 3bz8 coloured by chain, side view.
PDB entry 3bz8 coloured by chain, top view.
Source organism: Dictyostelium discoideum
Primary publication:
The small molecule tool (S)-(-)-blebbistatin: novel insights of relevance to myosin inhibitor design.
Lucas-Lopez C, Allingham JS, Lebl T, Lawson CP, Brenk R, Sellers JR, Rayment I, Westwood NJ
Org Biomol Chem 6 2076-84 (2008)
PMID: 18528569

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-140379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myosin-2 heavy chain Chain: A
Molecule details ›
Chain: A
Length: 762 amino acids
Theoretical weight: 86.75 KDa
Source organism: Dictyostelium discoideum
Expression system: Not provided
UniProt:
  • Canonical: P08799 (Residues: 2-759; Coverage: 36%)
Gene names: DDB_G0286355, mhcA
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand MG 2 x MG
Ligand VO4 1 x VO4
Ligand ADP 1 x ADP
Ligand EDO 1 x EDO
Ligand BL6 1 x BL6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: C2221
Unit cell:
a: 88.013Å b: 147.772Å c: 153.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.245
Expression system: Not provided

Quick links

Citations

3 review citations

Small-molecule inhibitors of myosin proteins.
Bond et al. (2013)
2 more

2 mentions without citation

The small molecule tool (S)-(-)-blebbistatin: novel insights of relevance to myosin inhibitor design.
Lucas-Lopez et al. (2008)
1 more