3c00

X-ray diffraction
1.41Å resolution

Crystal structural of the mutated G247T EscU/SpaS C-terminal domain

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-189559 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
T3SS structure protein EscU Chain: A
Molecule details ›
Chain: A
Length: 54 amino acids
Theoretical weight: 6.04 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AJ26 (Residues: 215-262; Coverage: 14%)
Gene names: ABE91_000815, BG944_004520, C0P57_005208, CTR35_004860, HKA49_004483, NCTC8009_02025, NCTC8179_06337, NCTC8960_02103, escU
T3SS structure protein EscU Chain: B
Molecule details ›
Chain: B
Length: 83 amino acids
Theoretical weight: 9.48 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AJ26 (Residues: 263-345; Coverage: 24%)
Gene names: ABE91_000815, BG944_004520, C0P57_005208, CTR35_004860, HKA49_004483, NCTC8009_02025, NCTC8179_06337, NCTC8960_02103, escU
Structure domains: secretion proteins EscU

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 26.969Å b: 53.884Å c: 59.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.216 0.236
Expression system: Escherichia coli BL21(DE3)