› 3c01

X-ray diffraction
2.6Å resolution

Crystal structural of native SpaS C-terminal domain

Released:
DOI: 10.2210/pdb3c01/pdb
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PDB entry 3c01 coloured by chain, front view.
PDB entry 3c01 coloured by chain, side view.
PDB entry 3c01 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Structural analysis of the essential self-cleaving type III secretion proteins EscU and SpaS.
Zarivach R, Deng W, Vuckovic M, Felise HB, Nguyen HV, Miller SI, Finlay BB, Strynadka NC
Nature 453 124-7 (2008)
PMID: 18451864

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154164 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Surface presentation of antigens protein SpaS Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 48 amino acids
Theoretical weight: 5.71 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40702 (Residues: 211-258; Coverage: 14%)
Gene names: STM2887, spaS
Surface presentation of antigens protein SpaS Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 98 amino acids
Theoretical weight: 11.37 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40702 (Residues: 259-356; Coverage: 28%)
Gene names: STM2887, spaS
Structure domains: secretion proteins EscU

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand CYS 4 x CYS
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P3221
Unit cell:
a: 96.663Å b: 96.663Å c: 217.511Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.223 0.254
Expression system: Escherichia coli BL21(DE3)

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Citations

27 review citations

Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.
Büttner D. (2012)
26 more

5 mentions without citation

Timing is everything: the regulation of type III secretion.
Deane et al. (2010)
4 more

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