3c3o

X-ray diffraction
2.15Å resolution

ALIX Bro1-domain:CHMIP4A co-crystal structure

Released:
Source organism: Homo sapiens
Primary publication:
ALIX-CHMP4 interactions in the human ESCRT pathway.
Proc Natl Acad Sci U S A 105 7687-91 (2008)
PMID: 18511562

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-186721 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Programmed cell death 6-interacting protein Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 42.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WUM4 (Residues: 1-359; Coverage: 41%)
Gene names: AIP1, ALIX, KIAA1375, PDCD6IP
Sequence domains: BRO1-like domain
Structure domains: alix/aip1 like domains
Charged multivesicular body protein 4a Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.52 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9BY43 (Residues: 210-222; Coverage: 6%)
Gene names: C14orf123, CDA04, CHMP4A, HSPC134, SHAX2

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1, SSRL BEAMLINE BL11-1
Spacegroup: C2
Unit cell:
a: 120.668Å b: 62.681Å c: 76.124Å
α: 90° β: 122.13° γ: 90°
R-values:
R R work R free
0.219 0.214 0.284
Expression systems:
  • Escherichia coli
  • Not provided