› 3cd9

X-ray diffraction
1.5Å resolution

Development of a family of redox-sensitive green fluorescent protein indicators for use in relatively oxidizing subcellular environments

Released:
DOI: 10.2210/pdb3cd9/pdb
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PDB entry 3cd9 coloured by chain, front view.
PDB entry 3cd9 coloured by chain, side view.
PDB entry 3cd9 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Development of a family of redox-sensitive green fluorescent protein indicators for use in relatively oxidizing subcellular environments.
Lohman JR, Remington SJ
Biochemistry 47 8678-88 (2008)
PMID: 18652491

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 28.14 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-148, 149-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 51.174Å b: 62.353Å c: 69.618Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.193
Expression system: Escherichia coli

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Citations

32 review citations

Fluorescent protein-based redox probes.
Meyer et al. (2010)
31 more

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