› 3cfo

X-ray diffraction
2.6Å resolution

Triple Mutant APO structure

Released:
DOI: 10.2210/pdb3cfo/pdb
PDB entry 3cfo coloured by chain, front view.
PDB entry 3cfo coloured by chain, side view.
PDB entry 3cfo coloured by chain, top view.
Source organism: Escherichia phage RB69
Entry authors: Wang J, Klimenko D, Wang M, Steitz TA, Konigsberg WH

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174312 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 909 amino acids
Theoretical weight: 105.41 KDa
Source organism: Escherichia phage RB69
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38087 (Residues: 1-903; Coverage: 100%)
Gene name: 43
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand GMP 1 x GMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 82.307Å b: 116.424Å c: 198.769Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.235
Expression system: Escherichia coli

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