Function and Biology Details
Reaction catalysed:
(S)-2-hydroxymethylglutarate + NAD(+) = 2-formylglutarate + NADH
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- 3-hydroxyisobutyrate dehydrogenase-like, NAD-binding domain
- 6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily
- 6-phosphogluconate dehydrogenase, domain 2
- 3-hydroxyisobutyrate dehydrogenase-related, conserved site
- 6-phosphogluconate dehydrogenase, NADP-binding
- NAD(P)-binding domain superfamily
- 3-hydroxyisobutyrate dehydrogenase-related
Structure analysis Details
Assemblies composition:
Assembly name:
2-(hydroxymethyl)glutarate dehydrogenase (preferred)
PDBe Complex ID:
PDB-CPX-171096 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-(hydroxymethyl)glutarate dehydrogenase
Molecule details ›
Chains: A, B, C, D
Length: 301 amino acids
Theoretical weight: 30.9 KDa
Source organism: Eubacterium barkeri
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 301 amino acids
Theoretical weight: 30.9 KDa
Source organism: Eubacterium barkeri
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q0QLF5 (Residues: 1-301; Coverage: 100%)
Sequence domains:
- NAD binding domain of 6-phosphogluconate dehydrogenase
- NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
wwPDB Validation report is not available for this entry.
X-ray source:
EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup:
P212121
Expression system: Escherichia coli BL21(DE3)
