› 3co1

X-ray diffraction
1.4Å resolution

Crystal structure of microtubule binding domain of human EB3

Released:
DOI: 10.2210/pdb3co1/pdb
PDB entry 3co1 coloured by chain, front view.
PDB entry 3co1 coloured by chain, side view.
PDB entry 3co1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mammalian end binding proteins control persistent microtubule growth.
Komarova Y, De Groot CO, Grigoriev I, Gouveia SM, Munteanu EL, Schober JM, Honnappa S, Buey RM, Hoogenraad CC, Dogterom M, Borisy GG, Steinmetz MO, Akhmanova A
OpenAccess logo J Cell Biol 184 691-706 (2009)
PMID: 19255245

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194056 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Microtubule-associated protein RP/EB family member 3 Chain: A
Molecule details ›
Chain: A
Length: 132 amino acids
Theoretical weight: 15.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UPY8 (Residues: 1-130; Coverage: 46%)
Gene name: MAPRE3
Sequence domains: Calponin homology (CH) domain
Structure domains: Calponin-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 47.188Å b: 85.365Å c: 32.161Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.234
Expression system: Escherichia coli

Quick links

Citations

41 review citations

Control of microtubule organization and dynamics: two ends in the limelight.
Akhmanova et al. (2015)
40 more

7 mentions without citation

Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins.
Zhang et al. (2015)
6 more