› 3czl

X-ray diffraction
2Å resolution

Crystal Structure Analysis of Sucrose hydrolase(SUH) E322Q-glucose complex

Released:
DOI: 10.2210/pdb3czl/pdb
PDB entry 3czl coloured by chain, front view.
PDB entry 3czl coloured by chain, side view.
PDB entry 3czl coloured by chain, top view.
Source organism: Xanthomonas citri pv. glycines
Primary publication:
Crystal structures and mutagenesis of sucrose hydrolase from Xanthomonas axonopodis pv. glycines: insight into the exclusively hydrolytic amylosucrase fold.
Kim MI, Kim HS, Jung J, Rhee S
J Mol Biol 380 636-47 (2008)
PMID: 18565544

Function and Biology Details

Reaction catalysed: 
Hydrolysis of sucrose and maltose by an alpha-D-glucosidase-type action
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180417 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 644 amino acids
Theoretical weight: 70.42 KDa
Source organism: Xanthomonas citri pv. glycines
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6UVM5 (Residues: 1-644; Coverage: 100%)
Gene names: BIY41_17820, supH
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GLC 1 x GLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6C1
Spacegroup: P21212
Unit cell:
a: 96.8Å b: 119.1Å c: 55.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.203 0.248
Expression system: Escherichia coli

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Citations

1 review citation

Enzymatic synthesis using glycoside phosphorylases.
O'Neill et al. (2015)
12 other articles