› 3det

X-ray diffraction
2.8Å resolution

Structure of the E148A, Y445A doubly ungated mutant of E.coli CLC_Ec1, Cl-/H+ antiporter

Released:
DOI: 10.2210/pdb3det/pdb
Model geometry
Fit model/data
PDB entry 3det coloured by chain, front view.
PDB entry 3det coloured by chain, side view.
PDB entry 3det coloured by chain, top view.
Source organisms:
Primary publication:
Ion permeation through a Cl--selective channel designed from a CLC Cl-/H+ exchanger.
Jayaram H, Accardi A, Wu F, Williams C, Miller C
Proc Natl Acad Sci U S A 105 11194-9 (2008)
PMID: 18678918

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211178 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 473 amino acids
Theoretical weight: 50.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37019 (Residues: 1-473; Coverage: 100%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel
Fab fragment, Heavy chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 221 amino acids
Theoretical weight: 23.69 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab fragment, Light chain Chains: D, F

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 231.167Å b: 97.514Å c: 173.277Å
α: 90° β: 132.88° γ: 90°
R-values:
R R work R free
0.257 0.255 0.299
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
11 more

6 mentions without citation

Basis of substrate binding and conservation of selectivity in the CLC family of channels and transporters.
Picollo et al. (2009)
5 more

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO