› 3dfk

X-ray diffraction
1.8Å resolution

The crystal structure of teicoplanin pseudoaglycone deacetylase Orf2* bound to one of its products decanoic acid

Released:
DOI: 10.2210/pdb3dfk/pdb
PDB entry 3dfk coloured by chain, front view.
PDB entry 3dfk coloured by chain, side view.
PDB entry 3dfk coloured by chain, top view.
Source organism: Actinoplanes teichomyceticus
Primary publication:
Crystal structures of lipoglycopeptide antibiotic deacetylases: implications for the biosynthesis of A40926 and teicoplanin.
Zou Y, Brunzelle JS, Nair SK
Chem Biol 15 533-45 (2008)
PMID: 18559264

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180623 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LmbE family N-acetylglucosaminyl deacetylase Chain: A
Molecule details ›
Chain: A
Length: 273 amino acids
Theoretical weight: 30.3 KDa
Source organism: Actinoplanes teichomyceticus
Expression system: Not provided
UniProt:
  • Canonical: Q6ZZJ1 (Residues: 1-273; Coverage: 100%)
Gene names: FHX34_108192, tcp14
Sequence domains: GlcNAc-PI de-N-acetylase
Structure domains: LmbE-like

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand DKA 1 x DKA
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 41.53Å b: 64.263Å c: 50.375Å
α: 90° β: 99.01° γ: 90°
R-values:
R R work R free
0.179 0.176 0.22
Expression system: Not provided

Quick links

Citations

3 review citations

β-Lactam and glycopeptide antibiotics: first and last line of defense?
Jovetic et al. (2010)
2 more

1 mention without citation

Structure and function of the LmbE-like superfamily.
Viars et al. (2014)