› 3dlt

X-ray diffraction
1.9Å resolution

Snapshots of esterase D from lactobacillus rhamnosus: Insights into a rotation driven catalytic mechanism

Released:
DOI: 10.2210/pdb3dlt/pdb
PDB entry 3dlt coloured by chain, front view.
PDB entry 3dlt coloured by chain, side view.
PDB entry 3dlt coloured by chain, top view.
Source organism: Lacticaseibacillus rhamnosus HN001
Entry authors: Bennett MD, Delabre M-L, Holland R, Norris GE

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109502 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine aminopeptidase S33 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 251 amino acids
Theoretical weight: 27.33 KDa
Source organism: Lacticaseibacillus rhamnosus HN001
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B2CZF3 (Residues: 2-244; Coverage: 100%)
Sequence domains: Serine aminopeptidase, S33
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand BUA 2 x BUA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P3221
Unit cell:
a: 46.848Å b: 46.848Å c: 173.954Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.205 0.255
Expression system: Escherichia coli BL21(DE3)

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