› 3dtg

X-ray diffraction
1.9Å resolution

Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design

Released:
DOI: 10.2210/pdb3dtg/pdb
PDB entry 3dtg coloured by chain, front view.
PDB entry 3dtg coloured by chain, side view.
PDB entry 3dtg coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis
Entry authors: Castell A, Unge T

Function and Biology Details

Reaction catalysed: 
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106396 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Branched-chain-amino-acid aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 372 amino acids
Theoretical weight: 40.37 KDa
Source organism: Mycolicibacterium smegmatis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0R066 (Residues: 2-368; Coverage: 100%)
Gene names: MSMEG_4276, MSMEI_4176, ilvE
Sequence domains: Amino-transferase class IV
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
2 bound ligands:
Ligand OBZ 1 x OBZ
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P21
Unit cell:
a: 60Å b: 87.8Å c: 72.9Å
α: 90° β: 105.8° γ: 90°
R-values:
R R work R free
0.238 0.238 0.274
Expression system: Escherichia coli

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