› 3dwl

X-ray diffraction
3.78Å resolution

Crystal Structure of Fission Yeast Arp2/3 Complex Lacking the Arp2 Subunit

Released:
DOI: 10.2210/pdb3dwl/pdb
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PDB entry 3dwl coloured by chain, front view.
PDB entry 3dwl coloured by chain, side view.
PDB entry 3dwl coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Structure and biochemical properties of fission yeast Arp2/3 complex lacking the Arp2 subunit.
Nolen BJ, Pollard TD
J Biol Chem 283 26490-8 (2008)
PMID: 18640983

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-126958 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Actin-related protein 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 427 amino acids
Theoretical weight: 47.43 KDa
Source organism: Schizosaccharomyces pombe
UniProt:
  • Canonical: P32390 (Residues: 1-427; Coverage: 100%)
Gene names: SPAC630.03, act2
Sequence domains: Actin
Structure domains:
Actin-related protein 2/3 complex subunit 1 Chains: C, H
Molecule details ›
Chains: C, H
Length: 377 amino acids
Theoretical weight: 41.64 KDa
Source organism: Schizosaccharomyces pombe
UniProt:
  • Canonical: P78774 (Residues: 1-377; Coverage: 100%)
Gene names: SPBC14C8.06, arc1, sop2
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Actin-related protein 2/3 complex subunit 2 Chains: D, I
Actin-related protein 2/3 complex subunit 3 Chains: E, J
Actin-related protein 2/3 complex subunit 4 Chains: F, K
Actin-related protein 2/3 complex subunit 5 Chains: G, L

Ligands and Environments

1 bound ligand:
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P4222
Unit cell:
a: 218.967Å b: 218.967Å c: 315.049Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.325 0.324 0.344

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Citations

2 review citations

Actin dynamics at the leading edge: from simple machinery to complex networks.
Insall et al. (2009)
1 more

4 mentions without citation

Structure and biochemical properties of fission yeast Arp2/3 complex lacking the Arp2 subunit.
Nolen et al. (2008)
3 more

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