› 3e5w

X-ray diffraction
1.71Å resolution

Crystal Structure Analysis of FP611

Released:
DOI: 10.2210/pdb3e5w/pdb
PDB entry 3e5w coloured by chain, front view.
PDB entry 3e5w coloured by chain, side view.
PDB entry 3e5w coloured by chain, top view.
Source organism: Entacmaea quadricolor
Primary publication:
Trans-cis isomerization is responsible for the red-shifted fluorescence in variants of the red fluorescent protein eqFP611.
Nienhaus K, Nar H, Heilker R, Wiedenmann J, Nienhaus GU
J Am Chem Soc 130 12578-9 (2008)
PMID: 18761441

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184835 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Red fluorescent protein eqFP611 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 242 amino acids
Theoretical weight: 27.7 KDa
Source organism: Entacmaea quadricolor
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ISF8 (Residues: 1-231; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand NRQ 4 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 71.25Å b: 104.46Å c: 133.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.209
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
4 more

4 mentions without citation

Chromophore transformations in red fluorescent proteins.
Subach et al. (2012)
3 more