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Cytokinin-induced structural adaptability of a Lupinus luteus PR-10 protein.

Fernandes H, Bujacz A, Bujacz G, Jelen F, Jasinski M, Kachlicki P, Otlewski J, Sikorski MM, Jaskolski M
FEBS J 276 1596-609 (2009)
Related entries: 1btv, 1bv1, 1fm4, 1icx, 1ifv, 1xdf, 2flh, 2qim

Cited: 33 times
EuropePMC logo PMID: 19220853

Abstract

Plant pathogenesis-related (PR) proteins of class 10 are the only group among the 17 PR protein families that are intracellular and cytosolic. Sequence conservation and the wide distribution of PR-10 proteins throughout the plant kingdom are an indication of an indispensable function in plants, but their true biological role remains obscure. Crystal and solution structures for several homologues have shown a similar overall fold with a vast internal cavity which, together with structural similarities to the steroidogenic acute regulatory protein-related lipid transfer domain and cytokinin-specific binding proteins, strongly indicate a ligand-binding role for the PR-10 proteins. This article describes the structure of a complex between a classic PR-10 protein [Lupinus luteus (yellow lupine) PR-10 protein of subclass 2, LlPR-10.2B] and N,N'-diphenylurea, a synthetic cytokinin. Synthetic cytokinins have been shown in various bioassays to exhibit activity similar to that of natural cytokinins. The present 1.95 A resolution crystallographic model reveals four N,N'-diphenylurea molecules in the hydrophobic cavity of the protein and a degree of conformational changes accompanying ligand binding. The structural adaptability of LlPR-10.2B and its ability to bind different cytokinins suggest that this protein, and perhaps other PR-10 proteins as well, can act as a reservoir of cytokinin molecules in the aqueous environment of a plant cell.

Articles - 3e85 mentioned but not cited (5)

  1. Crystallographically mapped ligand binding differs in high and low IgE binding isoforms of birch pollen allergen bet v 1. Kofler S, Asam C, Eckhard U, Wallner M, Ferreira F, Brandstetter H. J Mol Biol 422 109-123 (2012)
  2. The strawberry pathogenesis-related 10 (PR-10) Fra a proteins control flavonoid biosynthesis by binding to metabolic intermediates. Casañal A, Zander U, Muñoz C, Dupeux F, Luque I, Botella MA, Schwab W, Valpuesta V, Marquez JA. J Biol Chem 288 35322-35332 (2013)
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  4. Structural and bioinformatic analysis of the kiwifruit allergen Act d 11, a member of the family of ripening-related proteins. Chruszcz M, Ciardiello MA, Osinski T, Majorek KA, Giangrieco I, Font J, Breiteneder H, Thalassinos K, Minor W. Mol Immunol 56 794-803 (2013)
  5. The landscape of cytokinin binding by a plant nodulin. Ruszkowski M, Szpotkowski K, Sikorski M, Jaskolski M. Acta Crystallogr D Biol Crystallogr 69 2365-2380 (2013)


Reviews citing this publication (4)

  1. Structural and functional aspects of PR-10 proteins. Fernandes H, Michalska K, Sikorski M, Jaskolski M. FEBS J 280 1169-1199 (2013)
  2. Pathogenesis related-10 proteins are small, structurally similar but with diverse role in stress signaling. Agarwal P, Agarwal PK. Mol Biol Rep 41 599-611 (2014)
  3. Ligand Binding of PR-10 Proteins with a Particular Focus on the Bet v 1 Allergen Family. Aglas L, Soh WT, Kraiem A, Wenger M, Brandstetter H, Ferreira F. Curr Allergy Asthma Rep 20 25 (2020)
  4. Role of Small Molecule Ligands in IgE-Mediated Allergy. Khatri K, O'Malley A, Linn C, Kowal K, Chruszcz M. Curr Allergy Asthma Rep (2023)

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  2. The RNA hydrolysis and the cytokinin binding activities of PR-10 proteins are differently performed by two isoforms of the Pru p 1 peach major allergen and are possibly functionally related. Zubini P, Zambelli B, Musiani F, Ciurli S, Bertolini P, Baraldi E. Plant Physiol 150 1235-1247 (2009)
  3. An Ethylene-Induced Regulatory Module Delays Flower Senescence by Regulating Cytokinin Content. Wu L, Ma N, Jia Y, Zhang Y, Feng M, Jiang CZ, Ma C, Gao J. Plant Physiol 173 853-862 (2017)
  4. Subcellular localization and functional analyses of a PR10 protein gene from Vitis pseudoreticulata in response to Plasmopara viticola infection. He M, Xu Y, Cao J, Zhu Z, Jiao Y, Wang Y, Guan X, Yang Y, Xu W, Fu Z. Protoplasma 250 129-140 (2013)
  5. Differential gel electrophoresis (DIGE) to quantitatively monitor early symbiosis- and pathogenesis-induced changes of the Medicago truncatula root proteome. Schenkluhn L, Hohnjec N, Niehaus K, Schmitz U, Colditz F. J Proteomics 73 753-768 (2010)
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  7. Cryoprotection properties of salts of organic acids: a case study for a tetragonal crystal of HEW lysozyme. Bujacz G, Wrzesniewska B, Bujacz A. Acta Crystallogr D Biol Crystallogr 66 789-796 (2010)
  8. Crystal Structure of Hyp-1, a Hypericum perforatum PR-10 Protein, in Complex with Melatonin. Sliwiak J, Dauter Z, Jaskolski M. Front Plant Sci 7 668 (2016)
  9. PR-10 proteins as potential mediators of melatonin-cytokinin cross-talk in plants: crystallographic studies of LlPR-10.2B isoform from yellow lupine. Sliwiak J, Sikorski M, Jaskolski M. FEBS J 285 1907-1922 (2018)
  10. A comparison of two class 10 pathogenesis-related genes from alfalfa and their activation by multiple stresses and stress-related signaling molecules. Bahramnejad B, Goodwin PH, Zhang J, Atnaseo C, Erickson LR. Plant Cell Rep 29 1235-1250 (2010)
  11. Structure-based analysis of thermodynamic and mechanical properties of cavity-containing proteins--case study of plant pathogenesis-related proteins of class 10. Chwastyk M, Jaskolski M, Cieplak M. FEBS J 281 416-429 (2014)
  12. Solution structure of the strawberry allergen Fra a 1. Seutter von Loetzen C, Schweimer K, Schwab W, Rösch P, Hartl-Spiegelhauer O. Biosci Rep 32 567-575 (2012)
  13. Specific binding of gibberellic acid by cytokinin-specific binding proteins: a new aspect of plant hormone-binding proteins with the PR-10 fold. Ruszkowski M, Sliwiak J, Ciesielska A, Barciszewski J, Sikorski M, Jaskolski M. Acta Crystallogr D Biol Crystallogr 70 2032-2041 (2014)
  14. Molecular Cloning and Expression Analysis of hyp-1 Type PR-10 Family Genes in Hypericum perforatum. Karppinen K, Derzsó E, Jaakola L, Hohtola A. Front Plant Sci 7 526 (2016)
  15. Site-directed mutagenesis of histidine 69 and glutamic acid 148 alters the ribonuclease activity of pea ABR17 (PR10.4). Krishnaswamy S, Baral PK, James MN, Kav NN. Plant Physiol Biochem 49 958-962 (2011)
  16. Crystallographic and CD probing of ligand-induced conformational changes in a plant PR-10 protein. Śliwiak J, Dolot R, Michalska K, Szpotkowski K, Bujacz G, Sikorski M, Jaskolski M. J Struct Biol 193 55-66 (2016)
  17. Evaluation of the allergenicity potential of TcPR-10 protein from Theobroma cacao. Menezes SP, dos Santos JL, Cardoso TH, Pirovani CP, Micheli F, Noronha FS, Alves AC, Faria AM, Gesteira Ada S. PLoS One 7 e37969 (2012)
  18. Improved Shoot Regeneration, Salinity Tolerance and Reduced Fungal Susceptibility in Transgenic Tobacco Constitutively Expressing PR-10a Gene. Agarwal P, Dabi M, More P, Patel K, Jana K, Agarwal PK. Front Plant Sci 7 217 (2016)
  19. Purification and Characterization of Pathogenesis Related Class 10 Panallergens. McBride JK, Cheng H, Maleki SJ, Hurlburt BK. Foods 8 E609 (2019)
  20. The Y-segment of novel cold dehydrin genes is conserved and codons in the PR-10 genes are under positive selection in Oxytropis (Fabaceae) from contrasting climates. Archambault A, Strömvik MV. Mol Genet Genomics 287 123-142 (2012)
  21. High temperatures promote the uptake of hydrophobic pollutants by Cucurbita pepo via altered gene expression levels of major latex-like proteins. Inui H, Katte N, Goto J, Iwabuchi A. J Pestic Sci 45 75-80 (2020)
  22. MLP-PG1, a major latex-like protein identified in Cucurbita pepo, confers resistance through the induction of pathogenesis-related genes. Fujita K, Asuke S, Isono E, Yoshihara R, Uno Y, Inui H. Planta 255 10 (2021)
  23. Halostachys caspica pathogenesis-related protein 10 acts as a cytokinin reservoir to regulate plant growth and development. Feng Y, Ren Y, Zhang H, Heng Y, Wang Z, Wang Y. Front Plant Sci 14 1116985 (2023)
  24. Leaf-specific pathogenesis-related 10 homolog, PgPR-10.3, shows in silico binding affinity with several biologically important molecules. Han JH, Lee JH, Lee OR. J Ginseng Res 39 406-413 (2015)


Related citations provided by authors (6)

  1. Lupinus luteus pathogenesis-related protein as a reservoir for cytokinin.. Fernandes H, Pasternak O, Bujacz G, Bujacz A, Sikorski MM, Jaskolski M J Mol Biol 378 1040-51 (2008)
  2. Structure of a yellow lupin pathogenesis-related PR-10 protein belonging to a novel subclass.. Pasternak O, Biesiadka J, Dolot R, Handschuh L, Bujacz G, Sikorski MM, Jaskolski M Acta Crystallogr D Biol Crystallogr 61 99-107 (2005)
  3. Crystal structures of two homologous pathogenesis-related proteins from yellow lupine.. Biesiadka J, Bujacz G, Sikorski MM, Jaskolski M J Mol Biol 319 1223-34 (2002)
  4. Crystal structure of Vigna radiata cytokinin-specific binding protein in complex with zeatin.. Pasternak O, Bujacz GD, Fujimoto Y, Hashimoto Y, Jelen F, Otlewski J, Sikorski MM, Jaskolski M Plant Cell 18 2622-34 (2006)
  5. X-ray and NMR structure of Bet v 1, the origin of birch pollen allergy.. Gajhede M, Osmark P, Poulsen FM, Ipsen H, Larsen JN, Joost van Neerven RJ, Schou C, Løwenstein H, Spangfort MD Nat Struct Biol 3 1040-5 (1996)
  6. Crystal structure of a hypoallergenic isoform of the major birch pollen allergen Bet v 1 and its likely biological function as a plant steroid carrier.. Marković-Housley Z, Degano M, Lamba D, von Roepenack-Lahaye E, Clemens S, Susani M, Ferreira F, Scheiner O, Breiteneder H J Mol Biol 325 123-33 (2003)

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